Abstract
TRH immunoreactivity and bioactivity have been demonstrated in pancreatic islets , but studies with isolated rat pancreas have failed to show any direct stimulatory effect of TRH on G and I. In order to study the in vivo effect, TRH"Roche" was injected intravenously into fasted rabbits, and blood samples were obtained from the ear vein. Compared to saline controls (n=5), the peak increases (mean±SEM) for G (256±30pg/ml) and FFA (2,4±0,2mmol/l), and for I (23±4μU/ml) and BS (1,8±0,3mmol/1), were found within 15 and 60 min. after injection of 11 nmol (n=5,p=0,005) and 22 nmol (n=5, p=0,05) of TRH respectively. The analogous tripeptide pyroGluHisGlyOH did not influence G and I. A significant increase of G was observed with 1, 1 nmol TRH. The increase of G was augmented by simultaneous injection of TRH and insulin, and was nearly abolished in fed rabbits. It is suggested that TRH may have physiologic significance in modulating G, I, BS and FFA. In contrast to the effects of an alanine load, the increases of G and I are not significant within the first 5 min. This may suggest an indirect effect by humoral or neural mechanisms, possibly via the brain.
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Knudtzon, J., Trygstad, O. The in vivo effect of thyrotropin-releasing hormone (TRH) on plasmalevels of pancreatic spesific glucagon (G), insulin (I), bloodsugar (BS) and free fatty acids (FFA) in rabbits. Pediatr Res 14, 1416 (1980). https://doi.org/10.1203/00006450-198012000-00050
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DOI: https://doi.org/10.1203/00006450-198012000-00050