Abstract
Pelvic cartilages from chick embryos were incubated with precursors of proteoglycan synthesis: 3H glucosamine, 3H serine and 35S, in the presence of sera from patients with isolated growth hormone deficiency (IGHD), acromegaly & Laron type dwarfism (LTD) as well as healthy male adult serum (NHS).
Using the bioassay of Hall, but incubating both 3H glucosamine and 35S, SM activity in IGHD (n=3) was 0.01 U/ml (3H glucosamine) and 0.55 U/ml (35S), and rose to normal values after hGH administration. No such effect was found in LTD patients. SM activity estimated by 3H glucosamine based bioassays appears to be a better indication of clinical status than values derived from assays using 35S.
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Silbergeld, A., Mamet, R., Tacoli, A. et al. Stimulation of 3H glucosamine incorporation Into chick cartilage as an Index of serum somatomedin activity. Pediatr Res 15, 91 (1981). https://doi.org/10.1203/00006450-198101000-00120
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DOI: https://doi.org/10.1203/00006450-198101000-00120
