Abstract
Pre-B cells contain intracytoplasmic IgM but lack surface immunoglobulin (slg) detectable by immunofluorescence and are postulated to be the precursors of sIgM+ B cells in human bone marrow. Studies of the differentiation capacity of pre-B cells have been hampered by their low frequency and by the presence of B lymphocytes in normal bone marrow. I prepared a marrow cell suspension enriched for lymphoid cells by centrifugation over a 15-35% discontinuous sucrose density gradient. B lymphocytes were depleted by incubation on petri dishes coated with anti-Ig or anti-IgM antibodies. The final cell population was deficient in B lymphocytes and enriched for small pre-B cells.
Results of preliminary experiments suggest that absolute numbers of sIgM+ cells significantly increase during a brief period of culture under appropriate conditions.
Conclusions: These results provide additional evidence that pre-B cells lack stable sIgM molecules. Human bone marrow enriched for pre-B and depleted of B cells could prove useful in exploring the regulatory events involved in bone marrow B lymphopoiesis in vitro.
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Pearl, E., Kattwinkel, J. 957 STUDIES OF PRE-B CELLS IN HUMAN BONE MARROW. Pediatr Res 15 (Suppl 4), 602 (1981). https://doi.org/10.1203/00006450-198104001-00982
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DOI: https://doi.org/10.1203/00006450-198104001-00982
