Abstract
Previous studies in LN have suggested an immunologic pathogenesis. We have characterized B and T cell subpopulations from peripheral blood lymphocytes(PBL) of 10 patients with LN. B lymphocytes displaying different isotypes were detected by staining with fluorescein(Fl) and Rhodamine(Rh) conjugated, affinity-purified goat anti-human μ, σ, γ, or α. T lymphocytes were evaluated using monoclonal mouse anti-human T cell reagents(OKT3 = total T, OKT4 = inducer T, OKT8 = suppressor/cytotoxic T). Plasma cells(PC) were stained after fixation with Fl or Rh goat anti-human μ, δ, γ, or α.
Before therapy all patients had elevated percentages of sIgM+ B lymphocytes and IgM and IgA PC in blood. After treatment percentages of PC and B lymphocytes returned to normal. Distribution of T cell subpopulations were normal in pre- and post-treatment populations.
We conclude that children with LN possess elevated numbers of B cells and PC which may indicate spontaneous activation of the humoral immune system. After treatment normal B and PC numbers are seen. Such elevations of B cells are not due to an abnormal distribution of T cell subpopulations.
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Aronson, A., Schiffer, M., Levitt, D. et al. 1480 LYMPHOCYTE SUBPOPULATIONS IN LIPOID NEPHROSIS (LN). Pediatr Res 15 (Suppl 4), 690 (1981). https://doi.org/10.1203/00006450-198104001-01509
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DOI: https://doi.org/10.1203/00006450-198104001-01509
