Abstract
We studied the effect of premature weaning of suckling rats (at 17 days of age) to isocaloric high carbohydrate (H-CHO) or high fat diet (H-F) on the response of their isolated hepatocytes to glucagon. Hepatocytes were isolated from fed animals 72 hours after initiating the experimental diets. The effect of glucagon (10−9 to 5 × 10−7M) on gluconeogenesis from 5mM 14C- lactate was determined during incubations of 30 minutes. Basal gluconeogenic rates were, x ± SD: 7 ± .1 (C), 1.8 ± 0.5 (H-CHO) and 20.5 ± 6.1 (H-F) μ Mole/gm of wet weight. Maximal stimulation of gluconeogenesis by glucagon over basal was 70% for control (unweaned) 67% for high fat and over 350% for high CHO group. Thus, premature weaning to high CHO diet restored sensitivity to glucagon to a level even greater than seen in cells from fed adult rats. Changes from control in basal gluconeogenic rates may represent enzymatic induction and repression secondary to the diets. We conclude that glucagon resistance in older suckling rats is not related to maturity of the animals but reflects a metabolic adaptation to their natural high fat diet (maternal milk). (Grant from Canadian MRC # MR-6328)
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Beaudry, MA., Boodhoo, A. & Schiff, D. 222 ROLE OF NUTRITION IN THE HEPATIC RESISTANCE TO GLUCAGON 0F SUCKLING RATS. Pediatr Res 15 (Suppl 4), 476 (1981). https://doi.org/10.1203/00006450-198104001-00231
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DOI: https://doi.org/10.1203/00006450-198104001-00231
