Abstract
Six insulin dependent diabetic dogs were mated and delivered at term. Pups were compared to those from 6 control litters and studied after 0,3,6,9 and 24 hrs of neonatal fasting. The fetal hepatic metabolic environment in the IDM (N=8) was characterized by elevated hepatic glucose (G)(7.85±.76vs4.74±.45μ mol/g) glycogen (Gly)(759±70vs519±18), pyruvate (py)(.201±.017vs.123±.012), citrate (.298±.027vs.179±.025), aspartate(Asp)(.995±.117vs.608±.529) concentrations and augmented energy charge (.745±.014vs.697 ±.010). Fetal fructose-1,6 diphosphate (FDP)(.0084±.0012vs.019± .003) and AMP(.198±.01vs.283±.033) were lower in IDMs. Fetal Gly synthase activity was not affected however active Gly phosphorylase was decreased. Hepatic G levels became equivalent to controls at 3,6,9 and 24 hrs. Gly content remained elevated at 3 and 6 hrs becoming equivalent to controls at 9 and 24 hrs. Gly content declined to 304±50 in controls and to 466±110 in IDMs by 24 hrs. At 3 hrs the cytoplasmic NAD/NADH ratio and at 3,6 and 9 hrs Py levels were elevated in IDMs. At 3,9 and 24 hrs, IDM FDP and ADP levels remained lower. Hepatic alanine and Asp levels were increased in IDMs by 6,9 and 24 hrs. These data suggests that maternal canine diabetes 1) ↑fetal substrate stores without attenuating neonatal glycogenolysis, and 2) during the fetal period inhibition of phosphofructokinase by ↑citrate and ↑AMP values and inhibition of pyruvate dehydrogenase by ↑citrate may explain the alterations of FDP and Py respectively at these times. *Deceased.
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Kliegman, R., Miettinen, EL., Kalhan, S. et al. 259 REGULATION OF HEPATIC METABOLISM IN THE PUP OF A CANINE DIABETIC MOTHER (IDM). Pediatr Res 15 (Suppl 4), 483 (1981). https://doi.org/10.1203/00006450-198104001-00270
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DOI: https://doi.org/10.1203/00006450-198104001-00270