Abstract
To monitor the maturation of the Vitamin D dependent active calcium transport mechanism in neonatal rat intestine, calciumbinding protein (CaBP) was measured in duodena of suckling and weanling rats. Duodena from pylorus to ligament of Treitz were removed from pups from 1 to 7 weeks old, weighed, rinsed and homogenized. The 30,000 xg supernate of each homogenate was assayed for calcium-binding activity using the Chelex-100 competitive radiocalcium binding assay, for CaBP by radial immunodiffusion using an antibody raised in chickens against purified rat intestinal CaBP, and for total protein.
The marked enhancement of calcium binding activity and immunoreactive CaBP noted at weaning (Wk 3), corresponds with 1) enhancement and localization of active calcium transport characterizing functional maturation of the neonatal rat intestine and 2) maturation of the vitamin D endocrine system.
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gleason, W., Lankford, G. & Park, M. 556 INTESTINAL CALCIUM-BINDING PROTEIN IN THE DEVELOPING RAT DUODENUM. Pediatr Res 15 (Suppl 4), 533 (1981). https://doi.org/10.1203/00006450-198104001-00569
Issue date:
DOI: https://doi.org/10.1203/00006450-198104001-00569
