Abstract
The fetus and newborn are afflicted by infections that suggest a relative impairment in immunity; MØ play an important role in the immune response by clearing and processing foreign material. We compared surface epitopes necessary for antigen (Ag) presentation on neonatal cord blood MØ, fetal tissue MØ from the placenta, and adult blood MØ. Blood MØ were isolated by conventional methods. Placental MØ were obtained by enzyme digestion, density gradient separation, and selective adherence. Surface Ag expression was determined by indirect fluorescence microscopy using monoclonal antibodies towards: 1) Monomorphic HLA-DR determinants 7.2, L112 or 12.2; 2) a MØ surface epitope associated with enhanced accessory cell function-MØ 120; and 3) a human MØ marker F13. Viability of blood MØ was (x̄ ± S.D.) 91±5.3 and of placental MØ was 84±8 (NS); non-specific esterase and F13 staining were 90±4 and 87±4 and were comparable for each MØ type. Comparable and persistent expression of HLA-DR and MØ 120 were found for each MØ type.
F.A.C.S. analysis of 0 and 96 hr. adult and cord MØ gave parallel but somewhat lower values. Thus, newborn MØ have the relevant surface phenotypes for Ag presentation; further investigation is needed to define functional correlates.
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Glover, D., Wilson, C. MEONATAL AND PLACENTAL MACROPHAGES (MØ) SURFACE ANTIGEN EXPRESSION. Pediatr Res 18 (Suppl 4), 255 (1984). https://doi.org/10.1203/00006450-198404001-00973
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DOI: https://doi.org/10.1203/00006450-198404001-00973
