Abstract
Groups of two to three month old cotton rats seronegative for RSV antibody were administered rabbit anti-RSV serum with RSV antibody titer of 1:1024 as determined by neutralization test or RSV antibody negative normal rabbit serum, via intraperitoneal (IP), subcutaneous (SC) or intranasal (IN) routes. Twenty-four hours after the administration of serum, all animals were challenged IN with 2×105 PFU of Long strain live RSV and sacrificed four days later. Quantitation of RSV replication in the lungs and nasal turbinates was performed in HEp-2 cell cultures. High levels of RSV replication was observed in the lungs (103.5 PFU/gm of tissue) and nasal turbinates (102.5 PFU/gm of tissue) of rats that received normal rabbit serum. However, animals that were treated with rabbit RSV antibody-rich serum exhibited 100 to 1000 fold reduction in the titer of virus recovered. The protective effect observed was more significant in the lungs than in nasal turbinates, regardless of the route of administration of immuneserum. Furthermore, passive administration of RSV antibody was associated with attenuation of pulmonary histopathology, and no immune complexes were demonstrated in the lungs. These results suggest that high levels of passively administered RSV antibody at the mucosal site or in the peripheral circulation may limit the replication of the virus in respiratory tract and thus modify the outcome of subsequent respiratory disease.
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Dhar, R., Wong, D., Hovey, K. et al. 1085 EFFECT OF PASSIVE IMMUNOPROPHYLAXIS ON THE OUTCOME OF EXPERIMENTALLY INDUCED INFECTION WITH RESPIRATORY SYNCYTIAL VIRUS (RSV). Pediatr Res 19, 291 (1985). https://doi.org/10.1203/00006450-198504000-01115
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DOI: https://doi.org/10.1203/00006450-198504000-01115
