Abstract
In order to evaluate myocardial nucleotide metabolism tissue samples were taken during open heart surgery. Nucleotides were extracted and determined by means of isotachophoresis. Concentrations of hypoxanthine, inosine, and adenosine in coronary sinus' blood were measured with HPLC.
In human hearts the concentrations of nucleotides and creatine phosphate (CP) were: CP 19.9 ± 7.4, ATP 20.8 ± 13.5, ADP 11.1 ± 5.6, AMP 12.0 ± 6.4, IMP 3.4 ± 2.1, NAD+ 9.2 ± 3.0, and NADH/H+ 5.9 ± 2.8 nmoles/mg protein. Potassium induced ischemic heart arrest resulted in a significant decrease of 71% CP. In contrast an increase of 20% AMP, 16% IMP and 44% NADH/H+ was observed. During reperfusion a further decrease of all nucleotides was observed. Concomitant with the decrease of tissue nucleotides an increase of hypoxanthine, inosine, and adenosine in the coronary sinus occured These ischemic degradation products probably might be reutilized by the heart.
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Havel, M., Mohl, W., Schopf, G. et al. PURINE NUCLEOTIDES IN HUMAN HEARTS DURING OPEN HEART SURGERY: 82. Pediatr Res 19, 757 (1985). https://doi.org/10.1203/00006450-198507000-00102
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DOI: https://doi.org/10.1203/00006450-198507000-00102
