Abstract
The human hypoxanthine phosphoribosyltransferase (HPRT) gene has been isolated and characterized as a set of overlapping genomic clones spanning more than 30kb of the human X chromosome. Restriction endonuclease mapping and DNA sequence analysis of exon-intron boundaries show that the HPRT gene is split into nine exons whose boundary sequences are consistent with the established consensus splice site sequences and whose boundary locations are the same as those defined in the mouse HPRT gene. Transcription orientation and some intron sizes of genomic clones have been determined by heteroduplex analysis. Characterization of 5' flanking sequences shows that the promoter region lacks typical eukaryotic regulatory elements such as TATA and CCAAT boxes and is very rich in G and C residues. These characteristic features common to a number of housekeeping genes presumably have some role in the expression of the human HPRT gene.
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Kim, S., Moores, J., Respess, J. et al. ORGANIZATION OF THE HUMAN HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE GENE: 101. Pediatr Res 19, 760 (1985). https://doi.org/10.1203/00006450-198507000-00121
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DOI: https://doi.org/10.1203/00006450-198507000-00121
