Abstract
Rat heart myoblasts (H9c2) were cloned and cultivated in Eagle's minimum essential medium supplemented with 10% FCS. Clones exhibiting high creatine kinase (CK) activity were investigated. Reduction of FCS to 2% for one week resulted in a differentiation of myoblasts towards myocytes. Surface morphology and ultrastructural changes of myoblasts and differentiated myocytes (transverse banding pattern) were investigated by means of electron and light microscopy.
Following enzyme activities (nmoles/mg prot./hr) were measured in myoblasts and myocytes:
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Müller, M., Rumpold, H., Schopf, G. et al. CHANGES OF PURINE METABOLISM DURING DIFFERENTIATION OF RAT HEART MYOBLASTS: 138. Pediatr Res 19, 766 (1985). https://doi.org/10.1203/00006450-198507000-00158
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DOI: https://doi.org/10.1203/00006450-198507000-00158
