Abstract
The reutilization of purine bases and purine nucleòsides was investigated in rat heart myoblasts. The cells were cultivated in Eagle's medium. Incubations were performed at 37° for 1 hour,14C-labeled adenine, hypoxanthine, adenosine and inosine were used as substrates (5.2 μmol/1). After the incubation cells were harvested, purine metabolites extracted with perchloric acid and separated by thin-layer chromatography. The radioactivity of metabolites was measured.
The total incorporation into rat heart myoblasts' purines was for adenine, hypoxanthine, adenosine and inosine was 1.4 ± 0.77, 0.64 ± 0.22, 4.95 ± 1.49, 1.55 ± 0.68 nmoles/mg protein/hr respectively. Thin-layer chromatographic separation showed that most of the radioactivity has been incorporated into adenine nucleotides; the amount of labeled adenine nucleotides was 52%, 21%, 30% and 34% of total intracellular radioactivity using the 4 substrates. Phosphate stimulated incorporation in all experiments.
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Schopf, G., Rumpold, H. & Müller, M. PURINE SALVAGE IN RAT HEART MYOBLASTS: 185. Pediatr Res 19, 774 (1985). https://doi.org/10.1203/00006450-198507000-00205
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DOI: https://doi.org/10.1203/00006450-198507000-00205
