Abstract
The effects of various nucleosides and nucleotides on the activity of DPD partially purified from rat liver was studied using 5-FU, uracil and thymine as substrates. 5-FU was found to have a Km similar to those of uracil and thymine. DPD showed the highest specific activity when utilizing 5-FU as substrate (Table).
Uridine was found to be a potent non-competitive inhibitor of DPD for all three substrates. Thymidine is much less potent as an inhibitor. Compared to 5-FU and uracil, thymine reduction is the most susceptible to inhibition by uridine and thymidine (Table).
Uridine may enhance 5-FU cytotoxicity more than thymidine.
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Tuchman, M., Ramnaraine, M. & O'Dea, R. THE EFFECTS OF URIDINE AND THYMIDINE ON THE DEGRADATION OF 5-FLUOROURACIL (5-FU), URACIL AND THYMINE BY RAT LIVER DIHYDROPYRIMIDINE DEHYDROGENASE (DPD): 213. Pediatr Res 19, 779 (1985). https://doi.org/10.1203/00006450-198507000-00233
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DOI: https://doi.org/10.1203/00006450-198507000-00233