Abstract
Whole-body metabolic activity can be determined using noninvasive markers. Thus, urinary 3-methylhystidine has been shown to represent whole-body turnover of actin + myosin enabling calculation of muscle protein turnover. Similarly, we have shown that by measuring (HPLC) in 24h-urine the quantitatively excreted one-way RNA catabolites pseudouridine (Ψ), dimethylguanosine (m22Guo) and 7-methylguanine (m7Gua), the whole-body turnover of ribosomal RNA (rRNA),transfer RNA (tRNA) and messenger RNA (mRNA), respectively, can to estimated within reasonable limits. We have investigated whether there is a quantitative linkage between whole-body turnover of the main RNA classes and muscle protein turnover (Table). Taking into account the difference in creatinine-derived muscle mass between preterms and adults our data indicate that the turnover of both muscle protein and of the three major RNA classes in the whole body is three- to fourfold higher in preterm infants than in adults. Thus specific urinary modified RNA catabolites can be used as noninvasive markers for whole-body metabolic activity equivalent to 3-methylhistidine.
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Schöch, G., Sander, G., Hülsemann, J. et al. WHOLE-BODY RNA TURNOVER IN PRETERM INFANTS AND ADULTS IS QUANTITATIVELY LINKED TO MUSCLE PROTEIN TURNOVER. Pediatr Res 19, 1081 (1985). https://doi.org/10.1203/00006450-198510000-00080
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DOI: https://doi.org/10.1203/00006450-198510000-00080
