Abstract
The presence of CaBP (28k-Da) was localized in the ratepiphyseal plate by indirect immunohistochemistry using a specific antiserum in the rabbit against rat kidney CaBP.
Tibial epiphyseal cartilage from 21 d old rats was fixed in Carnoy's solution and frozen. Cryosections were incubated with diluted antiserum followed by peroxidase conjugated protein A. A series of controls were performed including the non immune rabbit serum instead of the specific anti-CaBP antibody.
CaBP was found exclusively in the nuclei of the chondrocytes. It was not uniformly distributed throughout the epiphyseal cartilage : it was selectively located in the proliferative and maturing chondrocytes ; specific staining was first observed in the nuclei of the resting chondrocytes. CaBP was not detected in the hypertrophic chondrocytes.
The data show the exclusive intranuclear localization of CaBP in chondrocytes implicated in proliferative activity. These findings raise the possibility that CaBP 28k-Da may be involved in the mitotic activity of the chondrocytes acting as a regulator of the intranuclear calcium. Furthermore, the localization of 28k-Da CaBP, which is different from that of 9k-Da CaBP ( reference to preceeding summary) suggests a specific role for each protein in the epiphyseal cartilage.
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Balmain, N., Brehier, A., Cuisinier-Gleizes, P. et al. NUCLEAR LOCALIZATION OF VITAMIN D-DEPENDENT CALCIUM-BINDING PROTEIN (CaBP 28k-Da) IN THE EPIPHYSEAL CARTILAGE IN GROWING RAT. Pediatr Res 19, 1117 (1985). https://doi.org/10.1203/00006450-198510000-00271
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DOI: https://doi.org/10.1203/00006450-198510000-00271
