Abstract
Mouse SMG renin expression exhibits sexual dimorphism. In the mature male, renin is synthesized under androgen influence, as prorenin which is converted intracellularly to intermediate single chain polypeptide (1-C renin) and subsequently processed to mature enzyme consisting of two polypeptide chains (2-C renin). 2-C renin is secreted from storage granules while 1-C renin is secreted predominantly via a constitutive pathway. Previous studies in this laboratory documented increases in SMG renin mRNA in male mice at puberty, as androgen rises. In this study we examined androgen effect on post-translational processing of renin during ontogeny. SMS from male CD-1 mice (ages 18-45 days) were incubated in RPMI 1640 media at 37°C for various time periods. Secretion rates as well as tissue renin levels increased with age (<1 ng Al/h/mg protein at day 18 to 5×104 ng AI/h/mg protein at day 45 in media and <100 ng AI/h/mg protein at day 18 to 3×106 ng AI/h/mg protein at day 45 in tissue). Western blot analysis of tissue and secreted renins from pre-pubescent mice showed that both storage and secreted forms contained 1-C:2-C renin in a 1:1 ratio. With puberty, both the storage and secreted renins were primarily of 2-C form. These data suggest that androgen influences renin transcriptional rates as well as post-translational processing and secretion.
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Ingelfinger, J., Pratt, R., Roth, T. et al. 276 ANDROGEN REGULATES SUBMANDIBULAR GLAND (SMG) RENIN SYNTHESIS AND SECRETICN AT MULTIPLE SITES. Pediatr Res 19, 156 (1985). https://doi.org/10.1203/00006450-198504000-00306
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DOI: https://doi.org/10.1203/00006450-198504000-00306
