Abstract
Vitamin E deficiency in rabbits causes iron deficient erythropoiesis, severe myopathy and abnormal sequestration of iron in skeletal muscle. We have demonstrated that iron is sequestered as ferritin in macrophages. Our further study also demonstrated that iron is also sequestered as ferritin in macrophages in skeletal muscle of experimental ischemic myopathy. Human muscles were evaluated in this study for possible sequestration of ferritin in infiltrating macrophages. The muscle biopsy specimens were rapidly frozen in isopentane cooled to -160° C with liquid nitrogen and stored at -70° C. Sections of 6μm thickness were cut in cryostat. Localization of ferritin was studied by an immunoperoxidase method. Rabbit anti-ferritin antibody conjugated with peroxidase (Accurate Chemical and Scientific Co.) was used to bind the tissue ferritin and consequently produced a red brownish end product from 3-amino-9-ethyl-carbazole. We observed no ferritin in muscle cells but abundant staining of ferritin in mononuclear cells infiltrating muscle of myositis patients. Normal muscle stains minimally, usually at perivascular areas. This study suggested that similar iron sequestration as ferritin in macrophages probably also exists in the muscles of patients with myositis as in the rabbits. (Supported in part by a grant from Fleur de Lis Fund and Donald Ogle Fund)
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Chu, JY., Li, J., Demello, D. et al. 628 SEQUESTRATION OF IRON IN MONONUCLEAR CELLS IN SKELETAL MUSCLES OF PATIENTS WITH MYOSITIS. Pediatr Res 19, 215 (1985). https://doi.org/10.1203/00006450-198504000-00658
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DOI: https://doi.org/10.1203/00006450-198504000-00658
