Abstract
Direct amelioration of intestinal ischemia using an inert intraluminal oxygen carrier is an appealing concept. The perfluorocarbons are biologically inert substances known to reversibly bind oxygen such that passive local delivery of oxygen to tissues is theoretically feasible. 75-100 gram weanling male Sprague-Dawley rats (n=46) were divided into timed study groups from 0.5 to 5 hours. All underwent laparotomy with identical complete de-vascularization of two adjacent 5 cm segments of terminal ileum. Each intestinal segment was treated with a single intraoperative instillation of either; Superoxygenated FC-43 (initial pO2596) or physiologic saline. Timed sacrifice was performed and tissue taken for light and electron microscopy. Blinded (norwai) histologic evaluation was performed in triplicate using a scoring system to quantitate the ischemic injury. (0= normal to 6= full thickness necrosis).
The fluorocarbon treatment group has a significantly (p<.01) lower histologic score than the saline treated controls at comparable time points; indicating a cytoprotective effect in the treatment group. (Wilcoxian sign rank test). The utility of this preliminary observation will require further laboratory and clinical experience.
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Oldham, K., Gore, D., Lobe, T. et al. 709 Use of Superoxygenated Intraluminal Fluorocarbons in the Treatment of Experimental Intestinal Ischemia. Pediatr Res 19, 229 (1985). https://doi.org/10.1203/00006450-198504000-00739
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DOI: https://doi.org/10.1203/00006450-198504000-00739
