Abstract
The purpose of this investigation was to establish whether there is an elevated prostanoid content in the intestinal mucosa in rats suffering from an immediate type gastrointestinal allergic reaction. Ten rats of the Hooded-Lister strain were sensitized with 250 μg/0.5 ml ovalbumin(OA) intraperitoneally (i.p.) together with B. Pertussis adjuvant. On the 14th day a booster of 2.5 μg OA/0.5 ml was given i.p. This procedure has previously been shown by us to produce local intestinal hypersensitivity. Control rats were given adjuvant only. The challenge procedure was identical in test and controls. Five days after the booster the rats were challenged with OA. Twenty-five mg. OA was introduced intragastrically and 45 minutes later the rats were sacrificed and 5 cm. of proximal small intestine removed. Prostanoid content of scraped mucosa was determined by radioimmunoassay. It was found that the PGE2 content in the sensitized intestine was 691±310 pg/mg protein as compared to a level of 424±155 pg/mg protein in controls This difference was significant (p < 0.05). There was no significant rise of 6-Keto PGF1α or TXB2 in the sensitized rats. These results show that PGE2 participates in intestinal immediate type responses and may explain some of the clinical manifestations of food protein allergy.
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Branski, D., Sharon, P., Eran, M. et al. PROSTANOID CONTENT OF SMALL INTESTINAL MUCOSA IN RAT MODEL OF PROTEIN HYPERSENSITIVITY. Pediatr Res 20, 695 (1986). https://doi.org/10.1203/00006450-198607000-00062
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DOI: https://doi.org/10.1203/00006450-198607000-00062
