Abstract
Probes 7C22 and pJ3.11 known to be closely linked to the cystic fibrosis locus were made available to us by Prof. Williamson, London. In situ hybridizations were carried out on metaphase preparations of lymphocytes from a patient having an apparent deletion in band q22 of one of the chromosomes 7, but no CF. The site and number of grains were scored for the normal and the deleted homologue. The majority of the grains resulting from hybridization with 7C22 were found in the region q22-qter, with a clustering at the distal part of band 31 and at band 32. For pJ3.11 most of the grains were in the region q31-qter. The normal homologue was more frequently labelled than the deleted one. This difference might be due to random variation, although alternatively structural chromosome aberrations other than deletion might be involved in this patient. In summary, our preliminary results suggest the distal half of the long arm of chromosome 7 to be the location of these probes. Our studies are now being extended to some other probes linked to the cystic fibrosis locus.
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Van Der Hout, A., Buys, C. 154 LOCALIZATION BY IN SITU HYBRIDIZATION OF DNA PROBES WITH TIGHT LINKAGE TO THE LOCUS FOR THE CYSTIC FIBROSIS MUTATION. Pediatr Res 20, 1059 (1986). https://doi.org/10.1203/00006450-198610000-00209
Issue date:
DOI: https://doi.org/10.1203/00006450-198610000-00209
