Abstract
Dispersed cells obtained from 10 GH-secreting adenomas were studied in perifusion columns, eluted with Krebs Ringer buffer. Fractions were collected each 5 min for 5 hours and assayed by RIA for GH and SRIF contents. Basal GH secretion was variable (10 to 94 ng/ml/ 106 cells). Within the first 15 minutes of the perifusion SRIF was detected in 5 cases (5 to 41 pg/ml/106 cells). However in 5 cases a great release of SRIF was only observed after 90 minutes or more (7 to 25 pg/ml/ 1O6 cells). In 2 cases, during the perifusion, the variations of GH and SRIF levels were significantly negatively correlated (p<0.001). Conclusions: 1) SRIF is released in vitro from human pituitary GH-secreting cells, 2) origin of SRIF release: internalization or synthesis ? 3) the inverse correlation between SRIF and GH levels suggests a role of the intracellular SRIF in the regulation of GH secretion.
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Peillon, F., Liapi, C., Garnier, P. et al. IN VITRO SIMULTANEOUS RELEASE OF ENDOGENOUS SOMATOSTATIN AND GROWTH HORMONE FROM HUMAN ADENOMATOUS PITUITARY CELLS. Pediatr Res 20, 1184 (1986). https://doi.org/10.1203/00006450-198611000-00064
Issue date:
DOI: https://doi.org/10.1203/00006450-198611000-00064
