Abstract
The extraction step with diethyl ether for a specific RIA of 17-hydroxyprogesterone (17-OHP) in dried blood spots (PANG et al JCEM 45, 1003, 1977) is unpracticable for mass screening of CAH. Based on the observation that 21-desoxycortisol (21-DOF) is elevated in untreated CAH (FRANKS JCEM 39, 1099, 1974) we designed a RIA intentionally with an antiserum (AS) crossreacting with 17-OHP (100%) and 21-DOF (48%). Dried blood spots on filter paper of 4,25 mm diameter - approx. 10 μl whole blood - were eluated with buffer containing chloral hydrate to separate the steroids from protein binding, and incubated with 1,2,6,7-H3-17-OHP and AS 1 : 3000 in microtiter plates shaking at room temperature for 21 hours. With a standard curve of 0 - 10.000 pg 17-OHP/spot we found for 17-OHP/21-DOF calculated in ng/ml plasma: sensitivity 3; coeff.var. at level 100 interassay 9%, intraassay 5%; in 2.274 newborns at day 5 mean 133,24; +/- 2 SD 36,56 - 229,92; in 2 untreated CAH range 1.000 - 2.000. In 15 adults the coeff.corr. between 17-OHP/21-DOF in dried blood and both steroids determined separately in plasma with chromatographic purification was 0,89 (linear), in 8 children with CAH treated or untreated it was 0,79 (exponential).
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Broessler, C., Horak, B., Lichtwald, K. et al. SCREENING FOR CONGENITAL ADRENAL HYPERPLASIA (CAH): COMBINED RADIOIMMUNOASSAY (RIA) OF 17-HYDROXYPROGESTERONE AND 21-DESOXYCORTISOL IN DRIED BLOOD SPOTS WITHOUT SOLVENT EXTRACTION. Pediatr Res 20, 1200 (1986). https://doi.org/10.1203/00006450-198611000-00162
Issue date:
DOI: https://doi.org/10.1203/00006450-198611000-00162
