Abstract
Human B cells hybridomas were produced by in vitro stimulation of normal human B cells with the p-azophenylarsonate (ARS) haptan coupled to keyhole limpet hemocyanin followed by fusion with the HAT sensitive human B cell line LICR-LON-HMy2. ARS specific hybridomas were cloned and analyzed for VH and JH gene usage.
Southern blot analysis of hybridoma DNA using a human JH probe demonstrated limited variability of restriction fragment size suggesting that few germline VH genes are involved in the human anti-ARS response exhibited in these cell lines. Sequential hybridization analysis of DNA from several hybridomas using JH1+2, JH3, JH4, JH1-5 and JH variation appears to be a major contributor to diversity in the human anti-ARS response.
These findings are in direct contrast to the well studied murine anti-ARS response in which a single VH gene and a single JH gene is responsible for the majority of the response and diversity derives from junctional, n-segment and somatic mutational mechanims. Mechanisms for generating diversity appear to be used to different extents in the human versus the murine anti-ARS response.
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Wasserman, R., Warshaw, J. HUMAN ANTI-HAPTEN ANTIBODY DIVERSITY DERIVES FROM COMBINATORIAL MECHANISMS EMPLOYING FEW VH GENES AND SEVERAL JH GENES. Pediatr Res 21 (Suppl 4), 319 (1987). https://doi.org/10.1203/00006450-198704010-00914
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DOI: https://doi.org/10.1203/00006450-198704010-00914
