Abstract
Significant aerobic lactate production and glutamine oxidation occur in developing rat lung and intestine. In intestine, the inhibitory effect of ATP on PFK has been reported to be eliminated by the presence of NH4+, an end product of glutamine oxidation. The association between aerobic lactate production and glutamine oxidation may be mediated by activation of PFK by NH4+ despite the presence of high ATP associated with aerobic conditions. In order to determine if lung PFK is controlled in a similar manner we compared the effect of NH4+ on developing rat lung and intestine PFK activity in the presence of 2 mM ATP. PFK activity was determined on particle free tissue homogenatea by measuring the disappearance of NADH in the presence of 2 mM fructose-6-phosphate, 2 mM ATP, and excess aldolase, alpha-glycerol phosphate dehydrogenasa, and triose phosphate isomerase at 27° and pH8.0. PFK activity is expressed as μmoles fructose-6-phosphate utilized/min/gram wet tissue. Data are mean±sd, n≥4.
(NH4)2SO4 stimulated lung PFK activity in the presence of 2 mM ATP by 3.7 fold compared to 18 fold for intestinal PFK activity. There was no difference between suckling and adult rats for either lung or intestinal PFK. This suggests that there may be an association between aerobic lactate production and glutamine oxidation in developing rat lung.
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Reinersman, G., Kimura, R. AMMONIUM SULFATE DECREASES THE INHIBITORY EFFECT OF ATP ON LUNG PHOSPHOFRUCTOKINASE (PFK) ACTIVITY. Pediatr Res 21 (Suppl 4), 345 (1987). https://doi.org/10.1203/00006450-198704010-01068
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DOI: https://doi.org/10.1203/00006450-198704010-01068
