Abstract
It has previously been shown that cholecystokinin octapeptide (CCK-8)as well as pilocarpine can accelerate the release of IgA in the gastrointestinal tract. The purpose of this study was to ascertain if CCK-8-induced IgA release involves calcium-coupled secretory mechanisms in the intestine. Hooded Lister rats were immunized with ovalbumin and Freund's complete adjuvant and a booster injection given 14 days later. On the 21st day a 10 cm. long segment of intestine was isolated 10 cm. distal to the pylorus. This was perfused with saline 0.5 ml/2.5minutes. After a flush out period of 30 mins. aliquots were collected every 2.5 mins. After 10 minutes 1 u CCK was given I.V.(group 1). A second group of animals received a calcium channel blocker Verapramil I.V. at a rate of 2 mcg/min. for 20 minutes. After the first 10 mins. 20 ng CCk-8 were administered I.V. Group 3 was given Verapramil only as above.
Results: following CCK-8 there was a significant rise of IgA into the intestinal fluid which began at 2.5 minutes and persisted for 10 minutes. Prior administration of Verapramil completely inhibited the CCK-8-induced release of IgA. Conclusion: it is suggested that CCK-8-induced release of IgA into the gastrointestinal tract is related to calcium dependent secretory mechanisms.
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Freier, S., Eran, M. & Faber, J. 28. IS IMMUNOGLOBULIN ‘A’ (IgA) RELEASE IN THE INTESTINE CONTROLLED BY A CALCIUM DEPENDENT SECRETORY MECHANISM?. Pediatr Res 22, 100 (1987). https://doi.org/10.1203/00006450-198707000-00049
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DOI: https://doi.org/10.1203/00006450-198707000-00049
