Abstract
Leukotrienes have been demonstrated to play a major role in mediating allergen induced asthma. We assessed the contribution of macrophages and PMN in the production of LTC4 in 11 patients with extrinsic or mixed type asthma, aged 3 to 15 years, and 10 healthy non allergic children, aged 4 to 15 years. Cells were separated by percoll gradient centrifugation and Monocytes were further separated by adherence. Cells were stimulated with 2μM A23187. LTC4 was quantitated by an RIA of the supernatant. Granulocytes of asthmatic patients generate significant, higher (p<0.001) amounts of LTC4 (range from 0.75 to 19.10 ng/ 106 cells, geometric mean 10.57 ng, 106 cells) than controls (range 0,21 to 5.86 ng/106 cells, geometric mean 1,95 ng/106 cells) whereas monocytes of the same patients produce amounts of LTC4 comparable to controls. The reason for the higher production of LTC4 by PMN of asthmatic patients might be the higher proportion of LTC4 producing eosinophils or an in vivo prestimulation of the eosinophils of these patients.
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Schauer, U., Eckhart, A., Müller, R. et al. Enhancement of leukotriene C4 (LTC4) production on peripheral polymorph-onuclear (PMN) cells form children with asthma. Pediatr Res 22, 223 (1987). https://doi.org/10.1203/00006450-198708000-00060
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DOI: https://doi.org/10.1203/00006450-198708000-00060
