Abstract
To study the distribution of a natural porcine surfactant in the lungs of surfactant deficient rabbits a monoclonal antibody against surfactant apoprotein was prepared using hybridoma technology. This monoclonal antibody reacts specifically with the instilled porcine and not with the rabbit surfactant protein. In six surfactant deficient rabbits, natural porcine surfactant was instilled endotracheally, six other surfactant deficient rabbits did not receive surfactant (controls). The effects of surfactant instillation included the ability to re-establish spontaneous room air breathing. Porcine surfactant protein was found in both bronchi and alveoli, in upper, middle, and lower lung parts. There was, however, inhomogeneous focal distribution. It is concluded that 3-4 hours after instillation, porcine surfactant appears to be trapped in collapsed lung parts.
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Bambang Oetomo, S., De Ley, L., Schoots, C. et al. IMMUNOHISTOCHEMICAL STUDY OF THE DISTRIBUTION OF EXOGENOUS SURFACTANT IN THE LUNGS OF SURFACTANT DEFICIENT RABBITS USING A MONOCLONAL ANTIBODY. Pediatr Res 22, 230 (1987). https://doi.org/10.1203/00006450-198708000-00104
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DOI: https://doi.org/10.1203/00006450-198708000-00104
