Abstract
An anticholeraic effect has been demonstrated for insoluble methylated total caseins in rat jejunum (Ped. Res. 18: 1075- 1079, 1984). We studied the anticholeraic effect of native and soluble formaldehyde-modified β and κ purified caseins.
Methods: Anticholeraic effect was determined in vivo in isolated rat jejunal loops. The transepithelial fluxes for intact proteins (ELISA) and degraded products (isotopic measurment) were performed in isolated stripped rabbit ileum in Ussing chamber in vitro.
Results: Native β and κ caseins have no effect on cholera toxin induced secretion in vivo whereas the soluble formaldehyde-modified proteins significantly reduced this secretion. No intact transepithelial passage was detected for both native arid modified β and κ caseins. The transepithelial fluxes of degraded products were for native and modified β-casein 1892 and 240 ng/h.cm2 respectively, and for native and modified κ-casein 351 and 221 respectively.
Conclusion: Formaldehyde-modified caseins have anticholeraic properties. This effect is probably due to an interaction of the modified proteins with the luminal side of the intestinal mucosa.
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Kheroua, O., Tomé, D., Marcon-Genty, D. et al. Anticholeraic effect and intestinal transepithelial passage of native and soluble formaldehyde-modified caseins. Pediatr Res 22, 234 (1987). https://doi.org/10.1203/00006450-198708000-00128
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DOI: https://doi.org/10.1203/00006450-198708000-00128
