Abstract
We tested the hypothesis that IGF-I plays a role in ovarian TI cell cytodifferentiation.
Dispersed TI cells obtained from 21 day old hypox Sprague Dawley rats were cultured in serum-free medium in the absence and presence of increasing concentrations of LH, IGF-I or a combination of these hormones. The culture medium was changed every 48 hours and the androsterone (andro) concentration in the culture supernates, determined by RIA, was used as an index of TI cell differentiation.
In the absence of LH or IGF-I, TI cells produced less than 0.1 ng/ml of andro. In the presence of 0.1, 10, and 50 ng/ml of LH, andro synthesis increased progressively to peak levels of 0.4, 0.85 and 1.35 ng/ml, respectively. IGF-I alone did not increase andro synthesis above control values. However, 1, 10 and 100 ng/ml of IGF-I potentiated the andro response induced by 10 ng/ml of LH to peak levels of 32±11, 220±115, and 435±65 ng/ml (mean±SEM), respectively. Peak andro levels were obtained between 48 and 96 hours of culture. Andro synthesis subsequently decreased, probably because of substrate depletion.
These results suggest that IGF-I plays a role in LH-induced differentiation of normal ovarian TI cells.
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cara, J., Rosenfield, R. INSULIN-LIKE GROWTH FACTOR-I (IGF-I) POTENTIATES LH-INDUCED STIMULATION OF ANDROGEN SYNTHESIS BY RAT OVARIAN THECA-INTERSTITIAL (TI) CELLS. Pediatr Res 21 (Suppl 4), 245 (1987). https://doi.org/10.1203/00006450-198704010-00466
Issue date:
DOI: https://doi.org/10.1203/00006450-198704010-00466
