Abstract
Hypersensitivity reactions to ingested proteins occur in different parts of the intestine, including the colon. Using an animal model of intestinal anaphylaxis we examined the effect of IgE-mediated reactions on colonic function. Electrolyte transport was assessed in the proximal colon by in vivo marker perfusion and in vitro in short-circuited chambers, before (Ag-F) and after antigen (Ag) challenge. Rats sensitized (S) to egg albumin were compared to sham-treated controls (C). In vivo: Net fluxes of H2O, Na+, K+ and Cl− were similar in S (n=8) and C (nl=6) during the Ag-F period. Antigen challenge resulted in a significant (p<0.05) reduction in H2O, Na+, Cl− absorption in S, but not C. In vitro: Ag challenge of stripped proximal colon from S produced a significant (p<0.05) rise in Isc (Ag-F, 56±9 μA vs Ag, 77±11) whereas tissue from C (47±8 vs 48±7) was unaffected. Na+ and Cl− flux studies demonstrated that the increase in Isc following antigen exposure in S was due to stimulation of Cl− secretion (Jnet) secondary to an increase in Jsm Cl− flux (Table, μ Eq cm−2h−1, *p<0.05).
No changes in Na+ fluxes were observed following Ag challenge. Conclusion: Food protein initiated IgE reactions in the proximal colon leads to abnormalities of H2O and electrolyte transport which are secondary to Ag stimulated Cl− secretion.
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Patrick, M., Forbes, D., Perdue, M. et al. COLONIC INTESTINAL ANAPHYLAXIS: IN VIVO AND IN VITRO TRANSPORT STUDIES. Pediatr Res 21 (Suppl 4), 274 (1987). https://doi.org/10.1203/00006450-198704010-00643
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DOI: https://doi.org/10.1203/00006450-198704010-00643
