Abstract
All proteins and peptides which in vitro and/or in vivo are toxic for the celiac small intestine are able to damage the in vitro developing fetal rat intestine and to agglutinate K 562 (S) cells (S.Auricchio et al.,J.Ped.Gastroenterol, and Nutr.4, 923, 1985). The minimal concentration of peptic-tryptic gp agglutinating all the cells (MAC) was found to be 73 mg/l. Various amines were able to inhibit the agglutinating activity of gp. The minimal concentration(mM) of the amines completely preventing cell agglutination induced by a gp concentration four fold the MAC was: 1.3 for putrescine, 0.8 for spermidine, 1.2 for spermine, 3 for glycinethylester, 2.3 for histamine, 1.6 for serotonine and 2.5 for monodansylcadaverine. 10 mM spermine or spermidine were unable to protect the cells from the agglutinating activity of Wheat Germ Agglutinin and Concanavalin A.Spermidine 0.35 mM significantly protected the in vitro developing fetal rat intestine from the toxic activity of gp tested at a concentration of 0.1 mg/ml culture medium in 11 experiments. Spermidine and other amines are therefore able to prevent the activity of gp in these in vitro systems; this effect might be related to a possible regulatory role of amines in endocytosis and/ or in brush border membrane functions (A.Elgavish et al., Biochim.Biophys.Acta 777, 1,1984).
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de Ritis, C., Maiuri, L., Raja, V. et al. 41 AMINES PREVENT THE IN VITRO TOXICITY OF GLIADIN PEPTIDES (gp) ON CULTURES OF FETAL RAT INTESTINE AND INHIBIT THE AGGLUTINATING ACTIVITY ON K 562 (S) CELLS. Pediatr Res 24, 412 (1988). https://doi.org/10.1203/00006450-198809000-00064
Issue date:
DOI: https://doi.org/10.1203/00006450-198809000-00064
