Abstract
Isolated human lymphocytes were incubated for 48 hours in FCS/RMPI-1640-medium with the addition of different mitogens (PHA, OKT-3, -4, -8 and PWM). The IGF-1 receptor affinity did not change before and after incubation with these mitogens (1.24+/-0.21 versus 1.41+/-0.68 10(+9) M(-1);n=9). For PHA (1ug/ ml) and OKT-3 (25ng/ml) stimulated lymphocytes we could demonstrate an increase of IGF-1 receptors / cell from 1640+/-350 to 2910+/-870 for PHA (n=7) and from 1770+/-390 to 2780+/-1280 for OKT-3 (n=9). In contrast, B-cells stimulated by PWM (10ug/ml) did not show any effect on IGF-1-binding. Stimulation of helper- and suppressor T-cells by OKT-4 and OKT-8 also did not influence IGF-1 binding. A dose dependent down regulation of IGF-1 receptor number could be demonstrated by addition of IGF-1 to cultured cells. In contrast to PHA and OKT-3, IGF-1 itself had no effect on DNA- or protein synthesis. Our results support the previous observation that IGF-1 binding to lymphocytes is increased by stimulation of T- but not of B-cells.
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Harmann, K., Kronenwett, M., Baier, T. et al. 106 INSULIN-LIKE GROWTH FACTOR-1 RECEPTOR EXPRESSION ON MITOGEN-STIMULATED LYMPHOCYTES. Pediatr Res 24, 534 (1988). https://doi.org/10.1203/00006450-198810000-00127
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DOI: https://doi.org/10.1203/00006450-198810000-00127
