THE EFFECT OF CALCIUM AND CHLORIDE CHANNEL BLOCKERS ON THE MOVEMENT OF ANTIBODIES AND ALBUMIN IN THE RAT INTESTINE

Abstract

We have previously shown that chloecystokinin (CCK) accerlerates the movement of immunoglobulins across the intestinal mucosa. This study investigates some mechanisms of this phenomenon, Hooded-Lister rats (160-180 g) were sensitized with 250ng Ovalbumin with Freund's complete adjuvant. On the 14th day a booster injection was given. On the 21st day the rats were anaesthetised and O.SnCi radiodinated human serum albumin was given i.v. A segment of intestine 10 cm long was isolated 10 cm. distal to the pylorus and perfused with 0.9% NaCl at a rate of 0.5 ml/min. The calcium channel blocker, Verapamil (50 g/ml) was injected i.v. 0.025 ml (1 ng) every 30 seconds for 20 mins. Ten mins. after starting Verapamil, 20 ng of CCK-octapeptide (CCK-OP) was given i.v. A control group received CCK-OP only. While in the control group there was a 20% rise of IgA and a 15% rise of ICG (P < 0.01) above baseline. Verapamil prevented this change. A second group received 0.2 mg of the chloride channel blocker, Furosemide (F) i.v. and 0.5 mg in the perfusate. F. also prevented the CCK-induced rise of antibodies. The difference between the treated and the control groups remained significant for about 12.5 minutes. The loss of albumin at 2.5 minutes was 35% above the baseline after CCK and only 13% above if F. was added before the CCK (P < 0.001). It is concluded that 1) CCK provokes an increased shift of albumin, IgA and IgG into the lumen of the intestine. (2) CCK causes a rise of cystolic calcium. (3) CCK-induced translocation of albumin, IgG and IgA i s prevented by F.

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