Abstract
To assess whether serum and mucosal DAO activity reflects quantitative changes in the small bowel mucosal mass (SBMM), a 50% proximal enterectomy or a single transection was performed in 20 growing rats. Ten days after surgery, hyperplasia of SBMM was demonstrated in each segment of the remaining gut (A : duodenum; B : proximal and C : distal ileum). Mucosal weight, protein and DNA content per cm were +38 to +78% higher (p < 0.01) in the resected group than in transected controls. In segments B and C of resected rats, total DAO activity was also increased respectively by +141% (p < 0.05) and by +87% (p < 0.01) over the controls, whereas in segment A, changes in DAO were small (+38%, n.s.) and not significant. In the ileum (B and C), there were significant correlations between total DAO and either mucosal weight (r = 0.75, p < 0.01) or mucosal DNA concentration (r = 0.78, p < 0.01) but not between DAO and sucrose activity. Compared to transected rats, the mean value of serum DAO was 5 fold higher in the resected group (p < 0.005).
In conclusion : after jejunectomy : (1) intestinal DAO activity reflects accurately quantitative changes of the SBMM in the ileum but not in the duodenum (2) serum levels of DAO could be a useful marker for ileal mucosal adaptation.
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Buts, J., Theys, S. CHANGES IN SERUM AND INTESTINAL DIAMINE OXIDASE ACTIVITY (DAO) AFTER PROXIMAL ENTERECTOMY IN RATS: CORRELATION WITH MUCOSAL MASS PARAMETERS. Pediatr Res 26, 268 (1989). https://doi.org/10.1203/00006450-198909000-00029
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DOI: https://doi.org/10.1203/00006450-198909000-00029
