CONTRIBUTION OF COLONIC ACETATE (Ac) TO CIRCULATING ACETATE POOL IN AN INFANT PIG MODEL

Abstract

Ac results from colonic fermentation of carbohydrate and fatty acid metabolism, and can be an energy source for infants. The contribution of colonic Ac to the circulating Ac pool was studied in 4 weaned, fasted, anesthetized neonatal pigs (age 5-11 d, wt 1.7-2 kg). Baseline Ac entry rate was determined from the plateau enrichment of ³H-Ac in the portal vein during a 1-h infusion. Subsequently, ¹⁴C-Ac (1 mmol/kg, 185-243 dpm/nmol) was infused intracecally for 1 h. ³H-Ac dilution in the portal vein was used to calculate the Ac entry rate. The dilution of ¹⁴C-Ac provided a measure of endogenous Ac in the circulating Ac pool. Colonic infusion increased portal Δc. by 321% over baseline (% DOB). In 2 animals, no dilution of ¹⁴C-Ac indicated that all circulating Ac yas derived from the colonic infusion. In 1 animal, dilution of ¹⁴C suggested that endogenous production of Ac was not suppressed. The table shows values before (Baseline) and after 1 h of intracecal infusion (End and Portal Ac).

Colonic Ac may make a major contribution to the circulating Ac pool and thereby affect endogeous production.

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