Abstract
Trophic factors in natural milk are potential mediators of the rapid growth of intestine in neonates. To determine whether nursing stimulates growth and development of small bowel mucosa, litters of piglets were divided into suckled and artificially reared groups at birth. The latter animals were raised in an automated feeding device (Autosow) with an artificial diet simulating the nutritional composition of sow milk. At 2, 8, and 15 d of age, animals were killed and 10-cm segments of jejunum, mid-bowel, and ileum were removed. Mucosal homogenates were prepared for enzyme assay and measurement of mucosal mass. Mean body weight, total length of bowel, and circumference of bowel segments did not differ between the two feeding groups at any age studied. As anticipated, mean mucosal ornithine decarboxylase activity decreased (p < 0.001) and measurements of mucosal mass increased (p < 0.001) with age; however, mean values for each of these measures were never greater in the nursed animals in comparison to the artificially reared group in any segment at any age. In addition, levels of disaccharidase activity did not correlate with the feeding regimen. To investigate the possibility that unanticipated growth factors in the artificial diet might have accounted for the apparent lack of trophic effect of nursing compared to artificial rearing, we evaluated the effects of this diet and of sow colostrum on 3H-thymidine incorporation in Swiss 3T3 cells in vitro. Colostrum, but not artificial diet, stimulated greater incorporation of 3H-thymidine than culture medium alone (p < 0.005). The mitogenic activity of colostrum was not inhibited by the addition of neutralizing antibody against epidermal growth factor or IGF-I. In conclusion, the effect of nursing on growth and development of the small bowel mucosa in the piglet does not differ from that of rearing with an artificial diet, despite the finding that porcine colostrum has greater growth-promoting activity than artificial diet in vitro. Neither epidermal growth factor nor IGF-I alone in colostrum appears to be required for this mitogenic effect.
Similar content being viewed by others
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ulshen, M., Lecce, J., Stiles, A. et al. Effects of Nursing on Growth and Development of Small Bowel Mucosa in Newborn Piglets. Pediatr Res 30, 337–341 (1991). https://doi.org/10.1203/00006450-199110000-00009
Received:
Accepted:
Issue date:
DOI: https://doi.org/10.1203/00006450-199110000-00009
