Abstract
PC secretion in adult rat type II cells can be stimulated by several agents acting via different signal-transduction mechanisms. To determine if these mechanisms also operate in developing lung we have compared the response of type II cells isolated from 21 day fetal and 1, 7 and 14 day old rats with those from adults to 36 uM terbutaline, 10 uM N-ethylcarboxamido-adenosine (NECA, adenosine analog), 1 mM ATP, 10 uM tetradecanoylphorbol acetate (TPA) and 25 nM ionomycin. Fetal cells were isolated by trypsin/collagenase digestion and differential adhesion and those from newborns and adults by elastase digestion and panning on IgG-coated dishes. The cells were cultured for 18-20 h with 3H-choline, washed in fresh medium and incubated ± agonists for 90 min after which 3H-PC in cells and medium was measured. The cells were 81-97% type II cells. The rate of basal secretion (PC in medium as % of total in cells + medium) was the same in all 5 groups.
However, there was a developmental change in the response to the secretogogues. These data show that different signal-transduction pathways mature at different developmental stages in type II cells. Supported by NIH (HL-31175) and the DFG.
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Griese, M., Gobran, L. & Rooney, S. 166 DEVELOPMENT CHANGES IN THE RESPONSE OF TYPE II CELLS TO PHOSPHATIDYLCHOLINE (PC) SECRETAGOGUES. Pediatr Res 30, 655 (1991). https://doi.org/10.1203/00006450-199112000-00196
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DOI: https://doi.org/10.1203/00006450-199112000-00196
