Abstract
During puberty an Increase In LH secretion, as measured by RIA, Is observed. Other studies have reported an increasing ratio of bioactivity over Immunoactivity of LH during puberty. In order to study the relationship between In-vitro biological (bLH) and immunological (ILH) LH secretion during normal pubertal development, bLH and ILH was measured in 62 normal children (31 boys, 31 girls) in different stages of puberty. Plasma samples were derived at 1 hour intervals during 3 hours daytime and 3 hours nighttime. BLH was measured by the Mouse Leydig Cell Assay (MLCA) and ILH was measured by IRMA.
Results: Boys: mean bLH and mean ILH significantly increased during puberty, with a maximum at G4 (G1: day + night bLH <0.3 U/l and day + night ILH <0.3 U/l; G4: day bLH 1.36 U/l sd 0.63, night 3.57 U/l sd 1.43; day ILH 1.14 U/l sd 0.62, night 4.33 U/l sd 1.29; Kruskai-Wallls oneway anova.p<0.01). A correlation is found between bLH and ILH, in all samples studied during daytime and nighttime (R = 0.85. p<0.01). At daytime, the ratio of the In-vitro bioactivity and the Immunoactivity (B/l ratio) Is 1.3 sd 0.2 in stages G2-G4, significantly higher compared to B/l 0.7 sd 0.2 In G5 (K-W oneway anova, p<0.05). During night B/l ratio is <1 and does not differ in all stages. Girls: for both bLH and ILH a significant increase during puberty is seen, with a maximum at M4 (M1: day + night bLH. day + night ILH <0.3 U/l; M4: day bLH 1.87 U/l sd 1.03, night 2.63 U/l sd 1.29; day ILH 2.45 U/l sd 1.73, night 5.03 U/l sd 2.37; K-W oneway ANOVA, p<0.01). BLH significantly correlates with ILH (R = 0.87, p<0.01). In contrast to boys, no significant changes In B/l ratio are detected in girls during puberty. In conclusion: during normal puberty, the changing LH In-vitro bioactlvity significantly correlates with ILH. In girls the LH B/l ratio does not change. In boys an Increased B/l ratio is found in stages G2-G4 during the day, which may be due to the low daytime testosterone levels, associated with the day-night rhythm.
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schroor, E., van Weissenbruch, M. & van de Waal, H. THE RELATION BETWEEN BIOACTIVE AND IMMUNOACTIVE LH DURING PUBERTY. Pediatr Res 33 (Suppl 5), S85 (1993). https://doi.org/10.1203/00006450-199305001-00489
Issue date:
DOI: https://doi.org/10.1203/00006450-199305001-00489
