Abstract
Hypoglycemia is a common sign in newborn endotoxic shock. Our previous study showed that glucose production was decreased and membrane glucose transporter gene expression was altered in the liver of endotoxic suckling rats. TNFα, a key mediator of endotoxic shock, may contribute to altered liver glucose production and membrane glucose transporter gene expression. TNFα this study was performed to evaluate TNFα effects on isolated hepatocyte glucose production and glucose transporter mRNA abundance, Hepatocytes were isolated from 10 day old Sprague-Dawley rats and incubated in RPMI media for 3 hours with or without 4.5×105 unit/ml rMuTNFα. Glucose production and membrane glucose transporter GLUT1 and GLUT2 mRNA abundance were determined. Hepatocyte membrane mRNA abundance was expressed as percent of controls. TNF blunted hepatic glucose production (82.6 vs 0.8 μg/107 cells without and with TNFα, respectively. p<0.001). TNFα increased GLUT2 mRNA abundance to 207% (p<0.01) and decreased GLUT2 mRNA abundance to 19% (p<0.001). Therefore TNFα decreased liver glucose production and altered hepatocyte membrane glucose transporter gene expression.
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Goto, M., Gottschalk, M., Hofmann, C. et al. TNF ALTERS GLUCOSE PRODUCTION AND MEMBRANE GLUCOSE TRANSPORTER mRNA ABUNDANCE IN ISOLATED RAT BEPATOCYTES. Pediatr Res 35, 283 (1994). https://doi.org/10.1203/00006450-199402000-00174
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DOI: https://doi.org/10.1203/00006450-199402000-00174
