Abstract
35 infants, aged 13.4 ± 11.8 months with vitamin D deficiency rickets were included in the study. Calcemia, phosphorus, serum alkaline phosphatases (ALP) level and isoenzymes, osteocalcin, 25 and 1.25 dihydrovitamin D metabolites, biological activity of Gs protein were measured before and after treatment. Gs protein or stimulatory G protein transduce the signal form PTH receptor to adenylate cyclase. Quantitative analysis of Gs-unit biological activity was determined by a technic, we adapted and optimized from that of Levine to applicate in infants (Assay variation = 10 %). The procedure was based on the measure of cAMP generated in vitro. Normal range is 85-110 % of a Gs biological activity of control patients. We observed, as soon as 1 week after treatment a expected signifiant change in all parameter but ALP. Particularly, Gs-unit activity is constantly decreased but one (73 ± 15 %) and increased to normal range after treatment (98 ± 15 %) (p < 0.002). Low Gs-units activities observed were similar to those found in our experience about pseudohypoparathyroidism type Ia. Our results are consisting with a PTH renal resistance in infants with vitamin D deficiency rickets, which could be due to low biological activity of Gs protein, in relation with vitamin D metabolite or hyperparathyroidism.
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Marguet, C., Basuyau, J., Leroy, M. et al. 146 Gs-UNIT ACTIVITY IN VITAMIN D DEFICIENCY RICKETS. Pediatr Res 36, 27 (1994). https://doi.org/10.1203/00006450-199407000-00146
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DOI: https://doi.org/10.1203/00006450-199407000-00146
