Abstract
Determination of pre-transfusion (Tx) red cell volume (mass) [RCV] in fetuses with Rh haemolytic disease and in anaemic infants helps assessment of Tx need and allows blood volume calculation. Neonates' RCV can be measured at Tx using dilution of autologous HbF by donor adult Hb, provided at least 20% of the Hb is fetal. We report an analogous method, using 0.1 ml blood samples, based on FACS - determined falls in RhD positive cells at Tx with D negative cells.
Pre Tx RCV = vol of red cells transfused X post Tx percentage Rh D positive/Pre- minus post- Tx percentage RhD positive.
The methods give comparable results (Neither the ranked pair differences nor thi median of the two samples were significantly different; n=10, p < 0.05). Our new method is reliable after smaller-volume Tx than possible using the Hb F dilution method. This investigation is placing Tx schedules in these patients on a more rational basis than previously possible.
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Wardrop, C., Dixon, S., Dawson, A. et al. 249 RED CELL VOLUME DETERMINATION IN ANAEMIC FETUSES AND NEWBORN INFANTS INQUIRING TRANSFUSION, USING FLOW CYTOMETRY: DILUTION PRINCIPLE. Pediatr Res 36, 44 (1994). https://doi.org/10.1203/00006450-199407000-00249
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DOI: https://doi.org/10.1203/00006450-199407000-00249
