Abstract
The current study was performed in 30 anesthetized and mechanically ventilated newborn rabbits to investigate the role of the endothelium-derived relaxing factor nitric oxide (NO) in the renal vasoconstriction observed during hypoxemia. Renal blood flow (RBF) and GFR were determined by the clearance of p-aminohippuric acid and inulin, respectively. In nine newborn rabbits (group 1), acute hypoxemia induced a significant decrease in RBF (-17 ± 7%) and GFR (-11 ± 6%). A second group of nine animals was used to determine the role of NO in regulating renal hemodynamics of the immature kidney in physiologic conditions.Nω-Nitro-L-arginine methyl ester (L-NAME), a NO synthesis inhibitor, significantly increased the renal vascular resistance by 31 ± 9% and decreased RBF and GFR (-20 ± 6% and -13 ± 5%, respectively). Acute hypoxemia was induced in 12 additional newborn rabbits during L-NAME infusion (group 3) to define the role of NO in the renal vasoconstriction observed during hypoxemia. The changes in renal hemodynamics were greater in this group than in those induced by hypoxemia alone. The present results suggest that: 1) endogenous NO has a crucial role in maintaining basal renal perfusion, 2) the activity of NO synthase is maintained during acute hypoxemia, and 3) NO could blunt the effects of acute hypoxemia in the immature newborn rabbit kidney.
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Abbreviations
- RBF:
-
renal blood flow
- RPF:
-
renal plasma flow
- FF:
-
filtration fraction
- RVR:
-
renal vascular resistance
- MAP:
-
mean arterial blood pressure
- PAH:
-
p-aminohippuric acid
- Pao2:
-
partial arterial pressure in O2
- Paco2:
-
partial arterial pressure in CO2
- NO:
-
nitric oxide
- EDRF:
-
endothelium-derived relaxing factor
- L-NAME:
-
Nω-nitro-L-arginine methyl ester
- ANOVA:
-
analysis of variance
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Acknowledgements
The authors thank Dolores Mosig for technical assistance and Dr. Denis Semama for valuable discussion of the results.
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Supported by Grant 32-36574.92 from the Swiss National Science Foundation.
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Ballèvre, L., Thonney, M. & Guignard, JP. Role of Nitric Oxide in the Hypoxemia-Induced Renal Dysfunction of the Newborn Rabbit. Pediatr Res 39, 725–730 (1996). https://doi.org/10.1203/00006450-199604000-00026
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DOI: https://doi.org/10.1203/00006450-199604000-00026
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