BOUND NEONATAL LYMPHOCYTES AFFECT THE GROWTH OF CANDIDA ALBICANS.† 1792

In human neonates and preterm infants, fungal infections produce significant morbidity and mortality. Human adult lymphocytes activated by Interleukin-2 (IL-2) closely adhere and inhibit the growth of hyphae forms ofCandida albicans. We hypothesized that neonatal lymphocytes, have decreased adherence to hyphae and therefore decreased capacity to inhibit the growth of C. albicans.

Methods: Lymphocytes were isolated from 10 adult peripheral blood samples, and the cord blood of 10 term, and 10 preterm infants by differential centrifugation, and placed in RPMI-1640 culture medium with IL-2 (1000u/ml) for 7 days. The lymphocytes were then Labelled with [⁵¹Cr] Chromium. The labelled lymphocytes were incubated with the hyphae form ofCandida albicans for 1 hr. Binding of the lymphocytes was assessed by [⁵¹Cr] incorporation. Data are presented as the mean ± SD.

Log in or create a free account to read this content

Gain free access to this article, as well as selected content from this journal and more on nature.com

Access through your institution

or

Sign in or create an account

Continue with Google

Continue with ORCiD