The release of endogenous vasoconstrictors together with changes in the vascular responses are central to the pathophysiology of sepsis. In the present study the effects of in vitro incubation for 20 h with heat-killed Group B Streptococcus (GBS, 3 × 107 cfu ml-1) on the vasoconstrictor responses to noradrenaline (NA, 10-8M - 10-4M), the thromboxane A2 analog U46619 (10-10M - 10-6M) and endothelin-1 (ET-1, 10-11M - 3 × 10-9M) were evaluated on isolated pulmonary and mesenteric arteries from 10-17 days old piglets. The incubation with GBS reduced the maximal contractile response to NA and ET-1 (P < 0.01) in both arteries. The nitric oxide (NO) synthase inhibitor L-NAME (10-4M) completely reversed this hyporesponsiveness. GBS-treated mesenteric arteries also showed a significant reduction of the maximal contractions induced by U46619 (P < 0.05) and this effect was inhibited by 10-4M L-NAME. In contrast, the maximal contractile responses to U46619 were similar in control and in GBS-treated pulmonary arteries. Addition of L-NAME was unable to modify the contractile responses to U46619 in GBS-treated pulmonary arteries.
In conclusion, GBS-treated systemic arteries from neonatal piglets showed decreased responses to NA, U46619, and ET-1 due to enhanced NO release. GBS-treated pulmonary arteries also exhibited decreased responses to NA and ET-1 but not to U46619. Induction of NO synthase in vascular smooth muscle, leading the release of large amounts of NO may play a key role in the hypotension and loss of systemic vascular responsiveness that occurs in GBS sepsis. The absence of pulmonary hyporesponsiveness to U46619 may partially explain the coexistence during sepsis of pulmonary hypertension and lung NOS induction.