Abstract
Cells producing IgA specific to ovalbumin (OVA) were detected with an assay of plaque-forming cells (PFC). Non-T cells were separated on a polystyrene resin column and were further depleted of B cells that bound sheep erythrocytes (SRBC) by SRBC-rosette sedimentation. The cells were recombined with T cells separated on a polystyrene resin column, stimulated with OVA antigen, and then cultured for 5 d. The number of OVA-specific IgA-PFC from the lymphocytes of infants allergic to hen's eggs (7 ± 5 per 7 × 104 non-T cells, n = 9) was significantly less than that of PFC from lymphocytes of age-matched controls (110 ± 18 per 7 × 104 non-T cells, n = 7) and from those of children with atopic dermatitis who were not allergic to hen's eggs (90 ± 30 per 7× 104 non-T cells, n = 4). Patients' B cells added to the culture supernatant from OVA-stimulated normal T cells (82 ± 18 per 7 × 104 non-T cells, n = 4) were able to produce the specific IgA to levels comparable to those of normal B cells (92 ± 9 per 7 × 104 non-T cells, n = 6), but the patients' T cells did not cause normal B cells to produce the antibody (8 ± 2 per 7× 104 non-T cells, n = 4). This indicates that the patients' T cells were less able than were normal T cells to promote the production of OVA-specific IgA-PFC. Until the age of 6 y, the ability of the patients' lymphocytes to produce specific IgA was abnormally low; from that age on, it was normal. At the stage of allergen entry, this transiently low production of OVA-specific IgA may contribute to the onset of allergy to hen's eggs.
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Abbreviations
- PFC:
-
plaque-forming cells
- OVA:
-
ovalbumin
- β-LG:
-
β-lactoglobulin
- SRBC:
-
sheep erythrocytes
- PWM:
-
pokeweed mitogen
- sIg:
-
surface Ig
- TGF-β:
-
transforming growth factor-β
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Acknowledgements
The authors thank Professor J. Yata for critical review and helpful discussion, and M. Kobayashi for secretarial assistance.
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Supported in part by a grant from the Ministry of Education, Japan.
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Noma, T., Yamaguchi, K., Yoshizawa, I. et al. T Cell Mediation of Abnormally Low Production of Ovalbumin-Specific Immunoglobulin A in Patients Allergic to Eggs. Pediatr Res 39, 329–335 (1996). https://doi.org/10.1203/00006450-199602000-00023
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DOI: https://doi.org/10.1203/00006450-199602000-00023
