Sensibility and Specificity of Semiquantitative Colorimetric Bromophenol-Blue Tests in the Diagnosis of Microalbuminemia in Children With Insulin-Dependent Diabetes Mellitus

The purpose of this study was to evaluate the utility of the semiquantitative colonmetric bromophenol-blue test in the diagnosis of microalbuminemia in 82 insulin-dependent diabetes mellitus patients, compared with radioimmunoanalysis (RIA) quantitative measurement.

In all cases, the first morning urine was analyzed twice by bromophenol blue test (Microbumintest, Bayer), maximum 30 minutes after voiding, and then freezed (-35oC) until the RIA quantitative determination. Urines with positive reaction for ketones and/or pH equal or higher than 7.0 (Bililabstix, Ames) were excluded from the study.

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