The Interrelationship of Hyperoxia and Nitric Oxide on the Neutrophil CD18 Receptor and Lung Myeloperoxidase in Piglets ♦ 1647

Nitric oxide (NO) down regulates the neutrophil CD18 receptor and is purported to decrease lung inflammation. However, in clinical situations, NO is used in the presence of oxygen (O₂), a lung inflammatory gas.

Thirty piglets, 10-14 d old, were exposed in chambers to room air (RA), RA+ NO, O₂, or O₂ + NO for 4-5 d and sacrificed. Ten additional animals received RA + NO, or RA + O₂ for 3 d, then recovered 3 d. At sacrifice, 10 ml of right atrial blood was collected into heparinized tubes for neutrophil baseline and PMA-stimulated surface CD18 (DAKO clone MHM23) expression by flow cytometry. Lung sections fixed in 4% formalin were immunohistochemically stained using a polyclonal myeloperoxidase (MPO) antibody (DAKO) and scored on a 1-3 scale. MPO activity was also measured biochemically in lung homogenates, by spectrophotometer, and was expressed as units of MPO/lung/min.

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