Abstract 1772
Poster Session IV, Tuesday, 5/4 (poster 348)
Surfactant protein B (SP-B) is critical for surfactant function. SP-B mobilization from lamellar bodies to the airspaces of the lungs may facilitate postnatal adaptation. The effects of postnatal dexamethasone (Dex) and exogenous surfactant on postnatal mobilization to the airspaces of preterm rabbits were studied. Design: Fetuses were delivered at 29 days gestation (31-32 days fullterm gestation) and sequentially assigned to either no ventilation (necropsy with postmortem lavage at birth) or one of six study groups: (Groups 1-2) spontaneous breathing in the presence or absence of 0.5 mg/kg intravascular postnatal Dex, (Groups 3-4) mechanical ventilation in the presence or absence of 0.5 mg/kg postnatal Dex, (Groups 5-6) mechanical ventilation + 100 mg/kg exogenous Survanta in the presence or absence of 0.5 mg/kg postnatal dex. Animals from Groups 1-6 were necropsied with postmortem lavage after 8 & 16 hours. Spontaneously breathing rabbits were maintained in an isolette with 50% O2. Mechanically ventilated rabbits were continuously ventilated with 50% O2 in individual temperature-controlled plethysmographs by adjusting peak inspiratory pressures to regulate tidal volumes. At necropsy, airspaces were lavaged with saline and samples were quantified for phosphatidylcholine (PC) content and SP-B content using a solid-phase, adsorption-based ELISA. Results: PC airspace pools were unaltered by Dex, and neither SP-B nor PC contents were significantly changed by up to 16h of mechanical ventilation. Postnatal Dex significantly elevated SP-B content in airspace lavage at 16h in both spontaneously breathing (p<.001) and mechanically ventilated (p<.01) preterm rabbits. Surfactant-treated animals following 16h of mechanical ventilation had greater than predicted SP-B levels based on the estimated content of SP-B in exogenous surfactant. Conclusions: Early postnatal Dex mobilizes SP-B to the airspaces via either increased SP-B secretion or decreased reuptake and/or clearance. Increased recycling of surfactant following instillation of treatment doses may stimulate subsequent SP-B mobilization.
