Abstract
L-selectin, a surface adhesion glycoprotein expressed on leukocytes, has a well-established role in mediating inflammation and lymphocyte recirculation. Recent evidence suggests that L-selectin may also influence hematopoiesis. We observed that a greater proportion of CD34+ cells express L-selectin in cord blood compared with adult bone marrow, and we hypothesized that L-selectin expression is associated with enhanced clonogenic properties. To test this, we compared CD34+/L-selectin+ cells with CD34+/L-selectin- cells in hematopoietic clonogenic assays. From CD34+/L-selectin+ cell cultures, we observed a 3-fold increase of d 12-14 colony-forming unit-granulocyte/macrophage and multipotent progenitor cells, and a 5-fold enhancement of primitive d 21 high proliferative potential colony-forming cells compared with the progeny of CD34+/L-selectin-cells. We conclude that CD34+ cord blood cells expressing L-selectin are enriched in their clonogenic activity compared with cell fractions lacking L-selectin expression.
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Abbreviations
- CFU-GM:
-
colony-forming unit-granulocyte/macrophage
- BFU-E:
-
burst-forming unit-erythroid
- CFU-GEMM:
-
granulocyte-macrophage, erythroid, megakaryocyte (multipotential) colony-forming unit
- HPP-CFC:
-
high proliferative potential colony-forming cells
- IMDM:
-
Iscove's modified Dulbecco's medium
- FITC:
-
fluorescein isothiocyanate
- PE:
-
phycoerythrin
- MNC:
-
mononuclear cells
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Acknowledgements
The authors thank the Labor and Delivery staff at St. Luke's Episcopal Hospital (Houston, Texas) and Shands Hospital at the University of Florida (Gainesville, Florida) for their cooperation in acquiring cord blood samples. We also thank Dr. Robert Christensen for useful discussions and for his help in acquiring bone marrow samples and Pam Connolly, RN, and Tina Lipe, RN. We thank Jutta Simon, Min Shi, and Karen Ramirez (Houston) for their excellent technical assistance, and the staff at the Interdisciplinary Center for Biotechnology Research flow cytometry laboratory at the University of Florida.
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Supported in part by a Grant-in-Aid from the American Heart Association-Texas Affiliate, Inc., a National Institutes of Health Clinical Investigator Development Award, K08 HD01062, and a University of Florida Howard Hughes Medical Research Institue Research Resources Program grant (all, J.M.K.).Present address (A.B.D.): Yale Cancer Center, Yale University School of Medicine, New Haven, CT 06520-8032.
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Koenig, J., Baron, S., Luo, D. et al. L-Selectin Expression Enhances Clonogenesis of CD34+ Cord Blood Progenitors. Pediatr Res 45, 867–870 (1999). https://doi.org/10.1203/00006450-199906000-00015
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DOI: https://doi.org/10.1203/00006450-199906000-00015
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